FastQCFastQC Report
Wed 28 Jun 2017
G154-M11-Index11_GGCTAC-_ACB6A7ANXX_L007_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG154-M11-Index11_GGCTAC-_ACB6A7ANXX_L007_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20785092
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA5400.010.1861711
CGTATGC6200.09.22603244
ACGGCTA6550.08.73274432
TCTCGTA6503.6379788E-128.4619941
CGGCTAC6159.458745E-118.22768533
TCGTATG6554.1836756E-118.06126643
CTCGTAT7753.6379788E-127.664796442
ATCGGAA8157.075869E-106.7490862
CGTCTGA9606.6211214E-106.187264416
TCACGGC12159.767973E-105.431787530
CATCTCG11953.6488927E-95.3392139
GTCACGG12904.5038178E-95.11603529
Produced by FastQC (version 0.11.2)