FastQCFastQC Report
Wed 28 Jun 2017
G154-M11-Index11_GGCTAC-_ACB6A7ANXX_L007_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG154-M11-Index11_GGCTAC-_ACB6A7ANXX_L007_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20785092
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG3000.016.8665610
GCGTCGT3250.015.56890911
CGTCGTG3600.013.44416612
GTCGCCG2902.3000575E-710.62136544
GATCGGA5600.09.8285621
GTCGTGT8301.8189894E-127.4214813
GAGCGTC7203.4197E-107.33328729
ATCGGAA7406.1118044E-107.1404212
TCGTGTA9305.456968E-126.860023514
CGTGTAG9601.0004442E-106.41628715
GTGGTCG6101.1485961E-45.7707241
AAGAGCG11305.320544E-95.4517797
GGTCGCC6553.1800108E-45.374387343
GATCTCG8003.516483E-55.223470734
TCGGAAG12152.9622242E-85.07371473
AGAGCGT10501.0158838E-65.02880678
Produced by FastQC (version 0.11.2)