FastQCFastQC Report
Wed 28 Jun 2017
G154-M12-Index12_CTTGTA-_ACB6A7ANXX_L007_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG154-M12-Index12_CTTGTA-_ACB6A7ANXX_L007_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20530397
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA6400.012.7203941
TCGTATG7550.011.65574443
CGTATGC7200.011.61122544
CTCGTAT7350.010.77569542
TCGGTCG1450.00807180810.62066637
ATCGGAA8300.09.8083762
TCTCGTA8200.08.58559641
CGTCTGA10400.08.2497416
ACGTCTG12200.07.393083615
ACACGTC13300.06.94696313
TCGGAAG12750.06.7298393
ATCTCGT11350.06.39664840
AATCTCG13100.06.21410439
CGGAAGA15050.05.84721954
Produced by FastQC (version 0.11.2)