FastQCFastQC Report
Wed 28 Jun 2017
G154-M13-Index13_AGTCAA-_ACB6A7ANXX_L007_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG154-M13-Index13_AGTCAA-_ACB6A7ANXX_L007_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19676171
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA6100.013.3459591
ATCGGAA7300.011.4533932
CTCGTAT7150.011.07686744
TCTCGTA7250.010.62063643
CGTCTGA9550.08.75352616
TCGGAAG11100.07.532013
ATCTCGT10600.07.47177442
ACGTCTG11650.07.3644715
AATCTCG10850.07.096954341
ACACGTC13100.06.88514613
CGGAAGA13350.06.5918614
Produced by FastQC (version 0.11.2)