FastQCFastQC Report
Wed 28 Jun 2017
G154-M13-Index13_AGTCAA-_ACB6A7ANXX_L007_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG154-M13-Index13_AGTCAA-_ACB6A7ANXX_L007_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19676171
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG3500.020.74282612
GCGTCGT3800.019.6842311
AGCGTCG4300.017.3954110
GTCGTGT6200.012.41924213
GATCGGA6050.012.3731551
GTCGCCG4100.011.80555144
GAGCGTC7700.010.2857779
TGGTCGC5457.2759576E-129.28486242
TCGTGTA8300.09.01196614
ATCGGAA8850.08.7075082
AGAGCGT10550.07.92486438
GTGGTCG6251.1259544E-97.74420541
CGTGTAG9400.07.723019615
GGTCGCC6851.1459633E-107.708469443
GATCTCG7304.656613E-107.230327634
TCGGAAG11250.06.84990553
AAGAGCG10600.06.84981357
TCGGTGG10202.7885108E-95.82306238
CGGAAGA14109.094947E-125.46472454
Produced by FastQC (version 0.11.2)