FastQCFastQC Report
Wed 28 Jun 2017
G154-M14-Index14_AGTTCC-_ACB6A7ANXX_L007_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG154-M14-Index14_AGTTCC-_ACB6A7ANXX_L007_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20801431
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA5202.5465852E-119.3089521
CCGTATC5651.1110387E-87.78812138
ATCGGAA7451.0913936E-117.67881732
TCTCGTA6259.1768015E-97.39195143
CTCGTAT7209.2712617E-76.111070644
CGTATCT8251.7365164E-65.60036739
CGTCTGA7955.3826807E-65.534301816
TCGGAAG11101.1198972E-75.1535283
Produced by FastQC (version 0.11.2)