FastQCFastQC Report
Wed 28 Jun 2017
G154-M14-Index14_AGTTCC-_ACB6A7ANXX_L007_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG154-M14-Index14_AGTTCC-_ACB6A7ANXX_L007_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20801431
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG3051.8189894E-1112.98304912
GCGTCGT3154.2200554E-1011.87267811
AGCGTCG3205.493348E-1011.68722310
GATCGGA5252.0845982E-77.5486551
GTCGCCG4459.21082E-67.416375644
GTCGTGT7354.0326995E-96.88402513
GAGCGTC6751.3160285E-55.86654239
TCGTGTA7854.307307E-65.604828414
TGGTCGC5600.00142149885.50047942
ATCGGAA8154.6945835E-55.1329992
CGTGTAG9008.547657E-65.132853515
Produced by FastQC (version 0.11.2)