FastQCFastQC Report
Wed 28 Jun 2017
G154-M15-Index15_ATGTCA-_ACB6A7ANXX_L007_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG154-M15-Index15_ATGTCA-_ACB6A7ANXX_L007_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19456751
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA5950.014.4218411
ATCGGAA8400.010.4771622
CTCGTAT7000.010.05732744
TCTCGTA6600.09.66684343
CGTCTGA9750.09.02527316
TCGGAAG11850.07.79785163
ACGTCTG11500.07.651861715
ACACGTC13500.07.007067713
CGGAAGA13400.06.89561374
ATCTCGT11050.06.769459242
AATCTCG11203.6379788E-126.286023641
Produced by FastQC (version 0.11.2)