FastQCFastQC Report
Wed 28 Jun 2017
G154-M15-Index15_ATGTCA-_ACB6A7ANXX_L007_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG154-M15-Index15_ATGTCA-_ACB6A7ANXX_L007_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19456751
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG3850.019.4282710
GCGTCGT3900.019.1790411
CGTCGTG4100.017.1701112
GATCGGA5650.014.8085371
GTCGCCG4700.012.17102644
GAGCGTC7300.011.1505699
ATCGGAA8400.010.2229392
GTCGTGT7700.09.71388413
TCGTGTA8200.09.12155114
CGTGTAG8800.08.74925615
AGAGCGT10000.08.1404598
GTGGTCG7205.456968E-127.94481141
TGGTCGC6553.583409E-107.72566742
GATCTCG7752.2919266E-107.094309334
AAGAGCG11400.06.94793947
TCGGAAG12000.06.7890793
GGTCGCC7352.9091098E-86.58547443
CGGAAGA15400.05.8616524
TCGGTGG11309.094947E-105.64553838
TCTCGGT10108.072857E-85.44403136
Produced by FastQC (version 0.11.2)