FastQCFastQC Report
Wed 28 Jun 2017
G154-M9-Index9_GATCAG-_ACB6A7ANXX_L007_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG154-M9-Index9_GATCAG-_ACB6A7ANXX_L007_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20895360
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA6100.09.738841
CGATCAG6900.09.24645933
CTCGTAT6600.09.00003742
CGTATGC6901.8189894E-128.28973144
ACGATCA7650.08.05214832
GTCACGA8600.07.67415629
TCTCGTA6105.7334546E-97.574019441
TCGTATG7601.8189894E-117.526202743
ATCGGAA8550.07.46268032
CACGATC9900.06.88848931
CGTCTGA9651.1277734E-106.38314116
ACGTCTG12700.06.06269815
GATCTCG8051.7421917E-76.01270639
ATCTCGT10302.1067535E-85.55360740
TCGGAAG12451.8208084E-95.30137873
TCACGAT13901.0968506E-95.064429830