FastQCFastQC Report
Wed 28 Jun 2017
G154-M9-Index9_GATCAG-_ACB6A7ANXX_L007_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG154-M9-Index9_GATCAG-_ACB6A7ANXX_L007_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20895360
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG3850.012.00004410
CGTCGTG3552.0008883E-1111.77421312
GCGTCGT3502.0190782E-1011.31421911
GTCGCCG3757.528797E-99.97401444
GATCGGA5803.6379788E-129.110051
GAGCGTC7501.2732926E-117.6267319
TGGTCGC5252.1093547E-77.54333742
GTGGTCG6001.933744E-66.600340441
CGTGTAG7352.9165676E-86.58463315
GTCGTGT6954.9192386E-76.33065513
ATCGGAA7851.05033905E-76.17026852
GGTCGCC6953.2894259E-66.014799643
TCGTGTA8353.5565245E-75.79613714
GATCTCG7901.6058801E-45.011080334
Produced by FastQC (version 0.11.2)