FastQCFastQC Report
Tue 27 Jun 2017
G155-M2-Index19_GTGAAA-_ACB6A7ANXX_L008_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG155-M2-Index19_GTGAAA-_ACB6A7ANXX_L008_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21249678
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT3800.016.21100211
CGTCGTG4100.015.56183412
AGCGTCG4750.012.968863510
GATCGGA6850.09.965441
ATCGGAA9000.07.58480742
GAGCGTC9900.07.11140359
GTCGTGT10153.9108272E-106.06917213
TCGTGTA10551.6007107E-106.047328514
GATCTCG10454.962203E-95.681994434
CGTGTAG12252.0736479E-105.567260715
AGAGCGT14251.2732926E-115.4038588
TCGGAAG15505.456968E-125.25639723
CGGAAGA17300.05.2184884
TGGTCGC7851.4470902E-45.044567642
Produced by FastQC (version 0.11.2)