FastQCFastQC Report
Tue 27 Jun 2017
G155-M3-Index20_GTGGCC-_ACB6A7ANXX_L008_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG155-M3-Index20_GTGGCC-_ACB6A7ANXX_L008_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21336241
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG3750.014.66780612
AGCGTCG4100.013.95187810
GCGTCGT4300.012.79127111
GATCGGA7150.08.3151851
TCGTGTA9300.07.333559514
ATCGGAA9802.3646862E-116.51607562
GTCGTGT10105.2750693E-116.31723413
GAGCGTC9502.1169399E-85.7897619
GTCGCCG7203.696946E-55.50013144
CGTGTAG10604.015601E-85.396316515
AGAGCGT12651.4822945E-85.04385858
Produced by FastQC (version 0.11.2)