FastQCFastQC Report
Tue 27 Jun 2017
G155-M4-Index21_GTTTCG-_ACB6A7ANXX_L008_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG155-M4-Index21_GTTTCG-_ACB6A7ANXX_L008_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21926493
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG4150.018.5556612
GCGTCGT4800.015.58408411
AGCGTCG5750.013.3920110
GATCGGA6750.011.4174441
ATCGGAA9700.08.626182
TCGTGTA10250.07.941591714
GAGCGTC10650.07.85028659
GTCGTGT9800.07.633125313
CGTGTAG11850.06.497921515
AGAGCGT13550.06.4951488
GTCGCCG7952.0409061E-86.364999344
TGGTCGC8355.5764758E-86.05997842
AAGAGCG17700.05.7181037
GATCTCG10151.5279511E-85.633282734
GTGGTCG10108.031384E-85.445347341
TCGGAAG16701.8189894E-125.14220953
Produced by FastQC (version 0.11.2)