FastQCFastQC Report
Tue 27 Jun 2017
G155-M5-Index22_CGTACG-_ACB6A7ANXX_L008_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG155-M5-Index22_CGTACG-_ACB6A7ANXX_L008_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20485321
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACG4500.026.89013133
CGTACGT4950.024.44551534
ACCGTAC7350.016.76288432
CGTAATC6800.016.1769438
GATCGGA8000.015.9502311
CTCGTAT7700.015.42758844
GTACGTA8200.014.75681435
TCTCGTA8400.013.61834643
TACGTAA10700.012.33704936
ATCGGAA10850.011.9631012
CACCGTA11450.011.52880431
ACGTAAT11350.010.66131137
AATCTCG12050.010.22411441
ATCTCGT13300.09.59384242
CGTCTGA13900.09.17976416
ACGTCTG14800.08.62132515
TCGGAAG16600.08.0842573
ACACGTC15250.08.07827313
TCACCGT16550.07.975322730
GTCACCG16950.07.39774129
Produced by FastQC (version 0.11.2)