FastQCFastQC Report
Mon 11 Sep 2017
G160-M1-Index1_ATCACG-_ACB0DPANXX_L004_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG160-M1-Index1_ATCACG-_ACB0DPANXX_L004_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15195920
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG187460.12336206034251299No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA172670.11362918467588667No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA44500.034.7823521
GCGTCGT21050.034.49000511
CGTCGTG21600.033.6117912
ATCGGAA46300.033.365182
AGCGTCG24750.030.31172210
ACGATCT24450.030.23483537
TCGGAAG53550.029.1768953
TCTCGTA25350.029.07500341
CACGATC25850.028.59669936
TCACGAT26450.028.53015735
CGTGTAG26600.027.87200715
CATCACG28750.027.85410733
ATCTCGT26950.027.75657740
CGGAAGA56100.027.7336354
GAGCGTC27900.027.20449
AGAGCGT27950.026.683118
GTCGCCG20350.026.49016244
TGGTCGC19800.026.33672742
ACACGTC31350.026.1057913
CGATCTC28750.025.94293238
Produced by FastQC (version 0.11.2)