FastQCFastQC Report
Mon 11 Sep 2017
G160-M1-Index1_ATCACG-_ACB0DPANXX_L004_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG160-M1-Index1_ATCACG-_ACB0DPANXX_L004_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15195919
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG188240.12387536416849813No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA172030.11320802644446842No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA42800.035.4932941
ATCGGAA44550.034.1321452
GCGTCGT22450.034.10360311
CGTCGTG22850.033.50660312
AGCGTCG25800.030.4428910
ACGATCT22800.030.39596637
CACGATC23650.029.6750336
TCTCGTA23350.029.58772941
GTCGCCG20950.028.77793944
TCGGAAG53700.028.6859023
TCACGAT24350.028.64143435
AGAGCGT27700.028.1149888
CGTGTAG27450.027.89082515
CGGAAGA54850.027.6838974
CATCACG27300.027.47915333
ATCTCGT25400.027.11195840
ATCTCGG25100.026.64614135
GAGCGTC30000.026.3267759
ACACGTC28900.026.11160913
ACGTCTG29350.025.86041515
Produced by FastQC (version 0.11.2)