FastQCFastQC Report
Wed 30 Aug 2017
G160-M11-Index1_ATCACG-_ACB0DPANXX_L004_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG160-M11-Index1_ATCACG-_ACB0DPANXX_L004_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19792575
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGACATCGCCGCCGACCCCGTGCGCTCGGCTTCGTCGGGAGACGCGTG613510.30996977401879244No Hit
GTCGGCTGCTAGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAAAACCGCGG536980.2713037591116871No Hit
CCTACTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAA530330.26794391331092593No Hit
GTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGC516640.26102717812108833No Hit
CGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGCGC423900.21417122329964647No Hit
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG389960.19702337871651365No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG375040.18948519836352773No Hit
CGGGTGGGTAGCCGACATCGCCGCCGACCCCGTGCGCTCGGCTTCGTCGG370230.18705499410763884No Hit
CTCGGCTTCGTCGGGAGACGCGTGACCGACGGTCCCCCCGGGACCCGACG366880.1853624402080073No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA344210.17390865008721704No Hit
GGGTAGCCGACATCGCCGCCGACCCCGTGCGCTCGGCTTCGTCGGGAGAC322380.16287926154125978No Hit
CGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGA321420.1623942311700221No Hit
GCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCC310020.15663449551157443No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC290990.14701977888172713TruSeq Adapter, Index 1 (100% over 50bp)
CTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGT279850.14139140561548966No Hit
GCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGCGCG260750.1317413221877396No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC220750.1115317233861688No Hit
CTCCTACTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCG217650.10996547947904707No Hit
GTTCAACGTGACGGGCGAGAGGGCGGCCCCCTTTCCGGCCGCACCCCGTT210320.10626207049865921No Hit
GCGGCGACGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGC206630.10439773500921432No Hit
CCGACATCGCCGCCGACCCCGTGCGCTCGGCTTCGTCGGGAGACGCGTGA203720.10292748669640003No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAT41150.034.9661635
ACGATCT41600.034.1117737
CACGATC42850.033.11667636
GATCGGA46500.033.1037371
GATCTCG42950.032.9871839
ATCACGA48550.031.31330334
CGATCTC48800.029.25929538
CGTCTGA53600.028.77099416
TCTCGTA49950.028.58370641
CATCACG54050.028.4525733
ACGTCTG54650.028.0974515
ACACGTC56000.027.45938313
TCGGAAG57450.026.9194283
CGTATGC56850.026.66347344
CGGAAGA59850.025.8031984
GCCGGAT11150.025.6756381
TCGTATG61900.024.63034843
CACACGT63300.024.50118312
CACGTCT65050.023.63913214
ATCGGAA73500.020.9516322
Produced by FastQC (version 0.11.2)