FastQCFastQC Report
Wed 30 Aug 2017
G160-M11-Index1_ATCACG-_ACB0DPANXX_L004_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG160-M11-Index1_ATCACG-_ACB0DPANXX_L004_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19792575
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCGC316560.15993876491563122No Hit
GGAGGATTCAACCCGGCGGCGCGCGTCCGGCCGTGCCGGTGGTCCCGGCG219250.1107738634311099No Hit
GGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCG218380.11033430465717574No Hit
CCGGCCGTCGCCGGCAGTCGGAACGGGACGGGAGCGGCCGCGGGTGCGCG217070.10967244029642428No Hit
CGCCTCTCTCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCCAGTGCGC197990.10003246166807503No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGTA40850.035.43774814
CGTGTAG41800.034.79024515
CGTCGTG44000.032.9015612
GATCTCG38900.031.61421634
GATCGGA47850.030.6367321
ATCTCGG40750.030.44936835
AGAGCGT48150.030.4300388
AAGAGCG48900.030.1427467
CGTATGC20950.028.893411
ATCGGAA50850.028.830792
GTCGTGT51400.028.2931713
GTAGATC48900.026.17806231
GTAGGGA57700.025.4661718
TCGGAAG58650.025.3728683
GTGGTCG46500.025.12682741
TCTCGGT50500.024.30932436
TCGGTGG53350.023.71342538
TAGATCT56150.022.6824332
CTCGGTG54550.022.46457737
GTGTAGG70800.021.09853416
Produced by FastQC (version 0.11.2)