FastQCFastQC Report
Mon 11 Sep 2017
G160-M2-Index2_CGATGT-_ACB0DPANXX_L004_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG160-M2-Index2_CGATGT-_ACB0DPANXX_L004_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25775601
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT23600.029.83022311
GATCGGA48900.028.9953861
ATCGGAA52650.026.9167922
TCGTATG27100.025.89919943
CGTATGC27750.025.68913544
TCGTGTA27850.025.43577614
CGATGTA28550.025.1984434
CGTCGTG28100.025.05314312
TATCTCG27250.024.54393639
AGCGTCG31050.024.30250510
CACCGAT31150.023.02166631
TCTCGTA30150.022.25690741
TCGGAAG64950.022.0904793
CCGATGT33100.022.0664733
CGTGTAG32750.021.76446715
GAGCGTC36400.021.2742759
GTCACCG35500.021.00445629
ACCGATG34400.020.97593332
TGGTCGC25450.020.92136442
TCACCGA34700.020.79232230
Produced by FastQC (version 0.11.2)