FastQCFastQC Report
Mon 11 Sep 2017
G160-M2-Index2_CGATGT-_ACB0DPANXX_L004_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG160-M2-Index2_CGATGT-_ACB0DPANXX_L004_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25775601
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA48700.029.2500131
GCGTCGT23350.028.73676711
ATCGGAA54150.026.2931232
CGTATGC27550.026.19572444
CGATGTA28300.025.34279334
CGTCGTG26800.025.11953712
TATCTCG27400.024.97200239
TCGTATG29200.024.79059643
AGCGTCG30200.024.33125310
TCGTGTA29050.023.62784814
CGTGTAG30150.022.76580415
TCTCGTA30800.022.14482541
TCGGAAG66500.022.0392783
TCACCGA34550.021.71024130
CACCGAT33850.021.70486631
GTCACCG34350.021.64348629
CCGATGT34600.021.17282333
ATCTCGT33100.020.67168840
TGGTCGC26500.020.4249742
CGGAAGA73000.020.1676274
Produced by FastQC (version 0.11.2)