FastQCFastQC Report
Wed 30 Aug 2017
G160-M22-Index2_CGATGT-_ACB0DPANXX_L004_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG160-M22-Index2_CGATGT-_ACB0DPANXX_L004_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26215440
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC302470.11537857079644669TruSeq Adapter, Index 2 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG42000.033.156939
CGTATGC46100.031.97428544
CGATGTA47850.030.89768234
TCGTATG48000.030.84613843
GATCGGA50950.029.305621
CACCGAT50550.029.2027831
TCACCGA50950.029.10305230
CCGATGT50800.028.93019133
ATCTCGT49750.028.03538940
ATCGGAA53600.027.8684022
GTCACCG53700.027.817329
TCTCGTA50950.027.67733241
ACCGATG57100.026.12354732
CTCGTAT60500.024.90933642
TCGGAAG67450.022.537113
CGTCTGA69300.021.74500316
ACACGTC71300.021.35102313
ACGTCTG73650.020.55028215
CGGAAGA80650.019.5849364
GATGTAT79900.018.09077535
Produced by FastQC (version 0.11.2)