FastQCFastQC Report
Wed 30 Aug 2017
G160-M44-Index4_TGACCA-_ACB0DPANXX_L004_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG160-M44-Index4_TGACCA-_ACB0DPANXX_L004_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19646773
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG1502850.764934780892516No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT1226140.6240923127681071No Hit
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT733300.3732419568343361No Hit
CAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAAC719260.36609574508750115No Hit
GTCTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGCGCA612570.31179166166372463No Hit
CGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACG586660.298603745256282No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA572240.29126411752199716No Hit
GGAGTCTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGC507060.25808818577992426No Hit
AACGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCG498460.25371087659026753No Hit
CTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG476880.24272688446087304No Hit
GCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTAC473620.24106757888432873No Hit
CGAACGCCGGGTTAAGGCGCCCGATGCCGACGCTCATCAGACCCCAGAAA460560.23442017678933835No Hit
AAACGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTC460330.2343031092179871No Hit
CTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGCGCAAT444790.22639341331016546No Hit
CTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGCAAATCGGTCGTCCGACC439720.22381283684603065No Hit
CGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCT417900.2127066872508783No Hit
GAATGATTAGAGGTCTTGGGGCCGAAACGATCTCAACCTATTCTCAAACT412190.20980035754472248No Hit
GGAAACTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGCAAATCGGTCGTC406070.206685342167897No Hit
CAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGCTCGCTTGATCTTGATTT392770.1999157826071488No Hit
GTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACGATCT389580.1982921062914505No Hit
CAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGT387650.19730975667098102No Hit
GCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAAC356450.1814292861224589No Hit
CTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGC339360.17273065658161776No Hit
GTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTAC336240.17114260952676555No Hit
GGAAAGTTGAAAAGAACTTTGAAGAGAGAGTTCAAGAGGGCGTGAAACCG320130.162942789637769No Hit
TCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGC300910.15316001258832684No Hit
GTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAA289400.14730154412635602No Hit
TGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTA288760.14697579088433504No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC287010.14608505936318397No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG286960.1460596098911511No Hit
CTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTT286360.1457542162267564No Hit
GCGAATGATTAGAGGTCTTGGGGCCGAAACGATCTCAACCTATTCTCAAA278260.14163140175742855No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA278060.14152960386929703No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACA266690.13574239392901827No Hit
CCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGA266250.13551843857512885No Hit
CTTGACTCTAGTCTGGCACGGTGAAGAGACATGAGAGGTGTAGAATAAGT262510.13361481806706882No Hit
CGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAGC262160.1334366717628386No Hit
GGGAGGCCCCCGGCGCCCGGCCCCGTCCTCGCGTCGGGGTCGGGGCACGC258990.13182317523595352No Hit
CAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGC257220.13092226392598927No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATT257040.1308306458266709No Hit
CGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTG255900.13025039786432102No Hit
CGATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATG255620.13010788082093686No Hit
CCGAAGTTTCCCTCAGGATAGCTGGCGCTCTCGCTCTTTTTTTCCCCGAC253800.12918152003893973No Hit
GTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAAT253780.12917134025012658No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAAC252540.12854019334371095No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC251020.12776652939391114No Hit
CCGAAAGCCGCCGTGGCGCAATGAAGGTGAAGGGCCCCGCCCGGGGGCCC247980.12621920149431157No Hit
GACTAATCGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGA245490.12495181778707373No Hit
CTGAACGCCTCTAAGTCAGAATCCCGCCCAGGCGGAACGATACGGCAGCG240340.12233052216768626No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGG236980.12062031764707619No Hit
CGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTC231990.11808046033819396No Hit
TGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGT230130.11713373997857053No Hit
CGAAGTTTCCCTCAGGATAGCTGGCGCTCTCGCTCTTTTTTTCCCCGACG226500.11528610830898285No Hit
GTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGTCACCGCC221660.11282259941619928No Hit
GGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACGATCTCAACCTATT219480.11171300243556538No Hit
CGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGG219050.11149413697608254No Hit
CCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAG217080.11049142777798675No Hit
CCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCT215860.10987046066038428No Hit
CGCGATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCA213470.10865397589721222No Hit
CGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCGCGGCGGCGCGG213080.1084554700153557No Hit
CGGAACGATACGGCAGCGCCGAAGGAGCCTCGGTTGGCCCCGGATAGCCG211870.10783959279215981No Hit
CGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCATGTGAACAGCAGTT210980.1073865921899744No Hit
GTGGGAGGCCCCCGGCGCCCGGCCCCGTCCTCGCGTCGGGGTCGGGGCAC207680.1057069270358038No Hit
CGGCGCCCGGCCCCGTCCTCGCGTCGGGGTCGGGGCACGCCGGCCTCGCG206300.10500452160769609No Hit
GTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGTC206070.1048874540363448No Hit
CGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGA206060.10488236414193822No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAG203790.10372695811164512No Hit
GGCGAAGCCAGAGGAAACTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGC202470.10305509204997686No Hit
GTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCTC199100.10133979763496019No Hit
CGCAGGTTCAGACATTTGGTGTATGTGCTTGGCTGAGGAGCCAATGGGGC198390.1009784151320932No Hit
AGCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAA197040.10029127938720522No Hit
GTTTCCCTCAGGATAGCTGGCGCTCTCGCTCTTTTTTTCCCCGACGTACG196740.10013858255500789No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGCCA188750.037.6417661
TCGTGTA13500.033.0812414
GTAGGGA13900.032.9162618
GAGTGTA13500.032.74696727
CGTGTAG13700.032.59830515
ATCTCGG12300.032.01529335
GATCTCG12450.031.80586434
GTAGATC12900.031.20271131
AGTGTAG13750.031.1915828
TGTAGAT14050.030.21271730
TGTAGGG15000.030.21018617
TCACCCG194250.030.0298123
TAGATCT13550.029.54576132
GTGTAGG15500.029.23760616
CACCCGG200600.029.0910824
TCTCGGT13450.028.29683336
GTCGTGT16000.028.05079813
CTGCCAG259250.027.3987872
AAAGAGT17100.026.11124224
GTGGTCG14300.025.38831741
Produced by FastQC (version 0.11.2)