FastQCFastQC Report
Mon 11 Sep 2017
G160-M5-Index5_ACAGTG-_ACB0DPANXX_L004_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG160-M5-Index5_ACAGTG-_ACB0DPANXX_L004_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17676152
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGAT10300.017.7418371
CCGGATA13600.014.2395762
TACGTTC18700.010.93848227
CCTACGT20400.010.35077325
GATCGGA14350.010.1262751
CGGATAA17650.010.0995833
GCGTCGT7050.09.98604411
CTCGGTT15750.09.9251141
GCGTACG29350.09.8225973
GGGCGAT9650.09.582521
CCCTACG22850.09.52973324
CGATGCG11950.09.39215853
CGTACGT30800.09.3603294
CGGGTAT9700.09.3061461
CTACGTT22850.09.240926
ACCGCGA7900.09.19068240
GCCGTAT5551.0913936E-119.1241531
CGCGTAC2902.7922599E-59.1104751
TACGTCG32300.08.9901036
TCGCTAT9650.08.8938672
Produced by FastQC (version 0.11.2)