FastQCFastQC Report
Mon 11 Sep 2017
G160-M5-Index5_ACAGTG-_ACB0DPANXX_L004_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG160-M5-Index5_ACAGTG-_ACB0DPANXX_L004_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17676152
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGAT11450.017.882921
TACGTTC20000.013.19677427
CCGGATA16100.012.9848152
CCTACGT21900.012.35370425
GCGTCGT6500.012.18467511
CGGATAA19200.011.576093
CTACGTT23950.011.02070726
CCCTACG25050.010.62463124
CGCGGTT3553.0231604E-910.5434191
GATCGGA15150.010.4635921
CGGGCAT16300.09.9955111
GGGCGAT9200.09.8119861
CGTCGTG8250.09.60004712
CGATGCG11850.09.470953
GTCCGAT7700.09.4359171
ACGTTCT30600.09.41589328
ATCGGAA17200.09.0838082
GTCGGTT9500.09.0386151
GTCCGTT8350.08.9650631
CGACGTT11100.08.9258671
Produced by FastQC (version 0.11.2)