FastQCFastQC Report
Wed 30 Aug 2017
G160-M55-Index5_ACAGTG-_ACB0DPANXX_L004_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG160-M55-Index5_ACAGTG-_ACB0DPANXX_L004_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25873220
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG601550.23249908592745702No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT417220.16125553757901026No Hit
CAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAAC388060.14998519704930427No Hit
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT275080.10631842499696598No Hit
GGAGTCTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGC270800.10466420491921763No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCCGG94800.021.4117264
GACACGG98800.020.55263315
GGACACG97500.020.30769314
CGGACAG107950.018.72414619
CCGGACA108100.018.29673212
ACGGACA111900.018.04563518
ACACGGA113300.017.94070616
CACGGAC117050.017.38381617
GCCAGTA159550.017.358534
CGGACAC115100.017.27955813
TCACCCG120800.016.8571663
CCCGGAC118650.016.76255611
CCAGTAG164200.016.733335
CCTGCCA166750.016.6859991
TGCCAGT169600.016.4070683
CGGCCCG126600.015.6399948
GTAGCAT181500.015.6001828
TAGCATA181000.015.594879
GATTGAC131100.015.33336526
CAGTAGC182900.015.2390186
Produced by FastQC (version 0.11.2)