FastQCFastQC Report
Wed 30 Aug 2017
G160-M66-Index6_GCCAAT-_ACB0DPANXX_L004_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG160-M66-Index6_GCCAAT-_ACB0DPANXX_L004_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24592626
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG2440670.9924397662941729No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT1813790.7375340884702594No Hit
CAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAAC1176640.4784523621023635No Hit
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT1026050.4172185597422577No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA792120.32209655040498725No Hit
AAACGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTC691390.2811371180938546No Hit
CGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACG687340.2794902829815734No Hit
GGAGTCTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGC681680.27718878008391623No Hit
GTCTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGCGCA673860.2740089651263757No Hit
CTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGCAAATCGGTCGTCCGACC648320.2636237382701628No Hit
AACGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCG641620.26089934438070994No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACA587100.23873009738772916No Hit
GGAAACTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGCAAATCGGTCGTC585240.23797377311394075No Hit
CAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGCTCGCTTGATCTTGATTT535830.2178823847441099No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATT525020.2134867581851568No Hit
CGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCT522820.2125921810871275No Hit
GCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTAC516460.2100060400219155No Hit
CTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTT503650.20479716155566308No Hit
CTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG484000.1968069615664468No Hit
GCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAAC481820.1959205169874905No Hit
CGATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATG476120.19360274905168728No Hit
GTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACGATCT475880.19350515882281136No Hit
GTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTAC461700.18773920280005885No Hit
GAATGATTAGAGGTCTTGGGGCCGAAACGATCTCAACCTATTCTCAAACT458450.18641766845069738No Hit
GTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAA442260.179834394261109No Hit
CTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGCGCAAT437970.17808996891995185No Hit
CGAACGCCGGGTTAAGGCGCCCGATGCCGACGCTCATCAGACCCCAGAAA434000.1764756638839626No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG419700.17066091274677214No Hit
CAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGT410320.1668467613015381No Hit
ATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGC404250.16437854176288455No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC394100.16025128833334024No Hit
CCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCT383240.15583533047670467No Hit
CGCGATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCA374670.1523505460539269No Hit
AAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG372000.15126485475768223No Hit
CGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTG369520.15025642239263104No Hit
CGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTC365330.14855265964683886No Hit
CAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCATG360760.1466943790386598No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC358890.1459339885053349No Hit
TGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTA350050.1423394150750717No Hit
CCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGA345770.14059905599345104No Hit
GTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAAT344720.1401720987421189No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA337700.1373175845474981No Hit
CTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGC336480.1368215008840455No Hit
CGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAGC325910.13252346455396832No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAAC325360.132299820279461No Hit
AGCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAA320570.13035208196147902No Hit
GTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGTC310040.12607031066954785No Hit
GACTAATCGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGA309120.12569621479219015No Hit
CGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCATGTGAACAGCAGTT307640.1250944083807886No Hit
CCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAG298200.1212558593783356No Hit
CGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGG297140.12082483586746695No Hit
GCGAATGATTAGAGGTCTTGGGGCCGAAACGATCTCAACCTATTCTCAAA296850.12070691434090852No Hit
GACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATGGCCGT296300.12048327006640121No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAG294810.11987739739546317No Hit
GTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCTC293500.11934471739618209No Hit
CAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGC292480.11892995892345941No Hit
TCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGC290920.11829562243576591No Hit
GGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGT288710.1173969790782001No Hit
CGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGA285410.11605511343115615No Hit
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGG282820.11500195221120348No Hit
CTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGA279670.11372108045720696No Hit
GGAAAGTTGAAAAGAACTTTGAAGAGAGAGTTCAAGAGGGCGTGAAACCG273550.11123252962087089No Hit
GGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACGATCTCAACCTATT273460.11119593328504243No Hit
GTGAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCA272230.11069578336205332No Hit
GGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGG270600.11003298305760434No Hit
GGAAGACCCACTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCCTTCTGAT269550.10960602580627216No Hit
CCGGCCGTCGCCGGCAGTCGGAACGGGACGGGAGCGGCCGCGGGTGCGCG267280.10868298489148738No Hit
CCCATATCCGCAGCAGGTCTCCAAGGTGAACAGCCTCTGGCATGTTGGAA265170.10782500412928656No Hit
GTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGTCACCGCC261160.10619443405515132No Hit
CCGAAAGCCGCCGTGGCGCAATGAAGGTGAAGGGCCCCGCCCGGGGGCCC258310.10503555008724974No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGG252650.10273404718959252No Hit
CGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAA248770.10115633848943174No Hit
CGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCAT246230.10012350856716155No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGCCA289500.038.0002441
TCACCCG268850.031.440863
CACCCGG276750.030.552254
CTGCCAG392650.028.0466692
TCCTGCC40800.027.0281471
CCTCACC324650.026.8551831
TGCCAGT422400.026.077543
GGACACG323300.025.93345314
GACACGG333150.025.49688315
CTCACCC344950.025.0652312
CCCGGAC334250.025.03882411
CCGGACA338750.024.77115212
CGGACAC354350.023.72408313
ACGGACA360300.023.50526418
GCCAGTA472450.023.3203324
CGGACAG364050.023.29126719
ACACGGA369750.023.13280916
CCAGTAG481350.022.8121015
CGTGTAG3600.022.61134115
GAGTCTA134800.022.305772
Produced by FastQC (version 0.11.2)