FastQCFastQC Report
Mon 2 Oct 2017
CBF_CON_1_S4_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBF_CON_1_S4_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23305771
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTGTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAAT397170.17041701817116456No Hit
GGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCA257590.11052627265581559No Hit
GGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTC246390.10572059598457394No Hit
GGATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGG242360.1039914105394754No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATTC56150.018.5917117
CGTATCG23950.017.8579923
GGGTTCG54750.017.0801533
TGTCTTA413000.016.9029454
GTCCGGT32600.016.5136871
TAGGTAT378350.016.4405029
TTAGGTA381200.016.4121158
CGTTTCG49250.015.8978749
CTTAGGT395250.015.81036957
GTCTTAG396000.015.7978865
CCGTTAT13100.015.5495111
GGGCGAT131200.015.304011
GGTATAT126500.014.7224271
GTCCCGT63350.014.2398391
GTGTATC118850.014.213672
GTCGTTT89700.014.1930461
GGGTAAT163850.014.1636181
ACGTTCG16400.014.1451076
GGCGATC137350.013.9355712
GTATATC131500.013.8387692
Produced by FastQC (version 0.11.2)