FastQCFastQC Report
Mon 2 Oct 2017
CBF_CON_2_S3_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBF_CON_2_S3_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22495125
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC288810.1283878173604281TruSeq Adapter, Index 4 (100% over 50bp)
GTCTGTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAAT235610.10473824884280483No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGG51250.018.2475517
GGGCGAT146900.017.2811851
TCGATTC50700.016.8726317
GGGTTCG49650.016.644373
AGTTACG76650.016.3619826
TTACGGA58600.016.2061758
CTCCTAT81350.015.9159421
CGTATCG21500.015.8467843
GACCAAA185750.015.7678995145
GGCGATC158000.015.0050022
GTCCGTT37150.014.8807451
TCGGTTT78250.014.8245342
GTCCGGT33100.014.7236911
TTCGCGC27350.014.5785437
CCCGAAT67150.013.9737681
TTAGGTA276450.013.6886538
TAGGTAT275950.013.5562829
CGGGCTA24300.013.1709251
TCGATAA21000.013.1173483
TGTCTTA320200.013.1080764
Produced by FastQC (version 0.11.2)