FastQCFastQC Report
Mon 2 Oct 2017
G23Rik_CON_1_S10_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG23Rik_CON_1_S10_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26011741
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTGTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAAT459770.17675479699724828No Hit
CAGGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCC389180.1496170517767342No Hit
CTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTCAC382430.14702206976457285No Hit
CTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATT379600.14593409952836298No Hit
GGATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGG337490.1297452561902719No Hit
GGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTC330500.12705800815101148No Hit
CCAGCCTTTTCTGTTTTGTCAGCAACCATAGATAATTCGGAGCACTCTTC299130.11499806952560383No Hit
GGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCA298470.1147443379510814No Hit
GCCTTTTCTGTTTTGTCAGCAACCATAGATAATTCGGAGCACTCTTCATC293350.11277599603963456No Hit
CAGCCTTTTCTGTTTTGTCAGCAACCATAGATAATTCGGAGCACTCTTCA271600.1044143873337813No Hit
CATTAATCTTTTCTACATTAAAGTTCCTTCCCGTAGAACTAGCTTCTTCT262390.10087367854385448No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTAAA87650.027.626766145
TCGGTTT93200.018.5916672
CGAATGT99400.018.1593743
CTCGAAT131750.017.9466651
TGTCTTA506450.016.675444
TTAGGTA471850.016.4704518
CTCCTAT104250.016.4697481
GTCTTAG475500.016.2971535
TAGGTAT476850.016.1006979
CGGTTTG105000.016.01723
GTCGGTT126750.015.8994771
GTCGTTT93100.015.78529551
CTTAGGT488850.015.7642137
GGGTTCG55650.015.3710943
TTACCGG27950.015.0439148
TCTTAGG550550.014.5897426
TCGATTC57500.014.3731397
TATCATG90050.014.0883126
CCCGTAT62700.013.9240231
ACTAATC48450.013.9163562
Produced by FastQC (version 0.11.2)