FastQCFastQC Report
Mon 2 Oct 2017
G23Rik_CON_1_S10_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG23Rik_CON_1_S10_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26011741
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCT662890.2548426112654282No Hit
CTTTAATGTAGAAAAGATTAATGGGGAATGGCATACTATTATCCTGGCCT504710.1940316105715492No Hit
GGAAACCTTCCAGCTGATGGGGCTCTATGGCCGAGAACCAGATTTGAGTT468010.17992259726098303No Hit
CCAGATTTGAGTTCAGACATCAAGGAAAGGTTTGCACAACTATGTGAGGA302220.11618599462450438No Hit
CGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTAC289810.11141507213992329No Hit
GAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGAA265940.10223844686136156No Hit
GGGAAACCTTCCAGCTGATGGGGCTCTATGGCCGAGAACCAGATTTGAGT262430.10088905621503767No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCAC196700.050.6844373
CACGATC206250.049.805041
ACGATCA212100.047.380232
GCCACGA97100.037.895221
TCACAAG319250.031.7303716
CCACGAT134400.028.6275711
GATCACA379800.027.0515384
TCTAACG22450.023.2548395
ATCACAA525700.020.0136625
CCACTAG99450.019.1017323
GCGTCGG112400.018.061916145
CACAAGA612950.017.4027637
TAACGCG31300.017.1442287
GTTTCGG69700.015.2919084
CACTAGC131350.014.6282684
ACAAGAA732900.014.5544018
GTTGATT90250.014.399451
AACGCGT37800.014.3878498
CTAACGC36850.014.1678736
CGCCGTT21200.014.022327145
Produced by FastQC (version 0.11.2)