FastQCFastQC Report
Mon 2 Oct 2017
G23Rik_CON_2_S9_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG23Rik_CON_2_S9_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25975816
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTGTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAAT407060.1567073003596884No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATG355830.13698510953419135TruSeq Adapter, Index 7 (97% over 35bp)
CTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATT348270.13407471010727826No Hit
CTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTCAC346810.1335126488422924No Hit
CAGGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCC327490.1260749614179589No Hit
GGATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGG282210.10864336273401383No Hit
GGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTC278390.10717276408179055No Hit
GGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCA268140.10322678602281445No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAT140750.020.3641591
CGAATGT114750.020.2783893
GGGTTCG58850.018.5999413
TCGATTC64100.017.6431487
TCGGTTT100000.017.4000642
TATCATG95750.016.7322316
CTCCTAT93250.016.1487961
TTCGATT85250.015.1378872
AGCTTTA247600.015.1070674
TCTAGCG40100.015.0052517
GTCGGTT140450.014.8654771
CGGTTTG106800.014.8646263
ATCATGC112800.014.8461027
CTAGCGG42250.014.7564288
ACTAATC51250.014.712252
CGTTTAC76000.014.1165995
TGTCTTA484550.014.00291254
GTCGTTT108600.013.9332421
TTAGGTA448600.013.5423578
CCCGAAT70600.013.4984921
Produced by FastQC (version 0.11.2)