FastQCFastQC Report
Mon 2 Oct 2017
G23Rik_CON_2_S9_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG23Rik_CON_2_S9_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25975816
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCT583670.22469746474951935No Hit
GGAAACCTTCCAGCTGATGGGGCTCTATGGCCGAGAACCAGATTTGAGTT490280.18874479246388257No Hit
CTTTAATGTAGAAAAGATTAATGGGGAATGGCATACTATTATCCTGGCCT484290.18643880138356386No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT299820.11542274552606932Illumina Single End PCR Primer 1 (100% over 50bp)
CCAGATTTGAGTTCAGACATCAAGGAAAGGTTTGCACAACTATGTGAGGA292100.11245075034408927No Hit
GGGAAACCTTCCAGCTGATGGGGCTCTATGGCCGAGAACCAGATTTGAGT270410.10410067579782671No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGATC178700.051.6398121
CGATCAC184200.048.7701263
ACGATCA196200.046.0827982
GCCACGA87500.034.3756831
TCACAAG295750.030.8191326
CCACGAT122650.028.9883541
GATCACA355750.025.9860844
CCACTAG96350.019.1141153
GCGTCGG103400.018.862486145
ATCACAA507350.018.5081025
TCTAACG21700.017.7098945
GTTTCGG74250.016.503114
CACAAGA579150.016.3147327
TAACGCG25450.015.6711727
CACTAGC130000.014.1108344
ACAAGAA708100.013.5892478
TAATGTA741650.012.7776834
GGAAACC648500.012.7775641
CGCCGTT18300.012.678448145
TCGCCGG46950.012.663258145
Produced by FastQC (version 0.11.2)