FastQCFastQC Report
Mon 2 Oct 2017
G23Rik_CON_3_S8_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG23Rik_CON_3_S8_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30968970
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTGTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAAT501710.1620040963583871No Hit
CAGGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCC400730.12939726442306607No Hit
CTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTCAC371310.11989743281742983No Hit
GGATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGG331370.1070006525887041No Hit
GGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTC331180.10693930085501713No Hit
CTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATT322690.10419784707079377No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAT153850.017.82541
TCGGTTT122400.017.7106232
CGAATGT117750.016.2524593
GTCGGTT152400.016.228911
TGTCTTA546800.015.431234
CGGTTTG124950.015.3739593
TTAGGTA504000.015.0742938
GTCTTAG514700.014.8445875
TAGGTAT511000.014.7973649
GTCCGCT94050.014.7730441
CTCCTAT121500.014.6684821
ACTAATC60450.014.6321322
CTTAGGT520850.014.4753247
GGGTTCG61750.014.4392363
TCTAGCG40750.014.4100357
GTCCGTT55150.014.2453661
AGCTTTA283850.014.045914
GTTCGAT90300.013.8559441
GTCCGGT50600.013.8011761
TTCGATT82900.013.2933112
Produced by FastQC (version 0.11.2)