FastQCFastQC Report
Mon 2 Oct 2017
G23Rik_CON_3_S8_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG23Rik_CON_3_S8_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30968970
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCT659250.21287437070073692No Hit
CTTTAATGTAGAAAAGATTAATGGGGAATGGCATACTATTATCCTGGCCT509180.16441618820386986No Hit
GGAAACCTTCCAGCTGATGGGGCTCTATGGCCGAGAACCAGATTTGAGTT506020.16339581200149697No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGATC211300.051.5169451
CGATCAC208900.050.8479273
ACGATCA230850.046.1387632
GCCACGA105050.036.834241
TCACAAG356050.030.1618086
GATCACA441050.024.6919924
CCACGAT154650.024.6140692
GCGTCGG104200.023.588314145
ATCACAA635650.017.7382015
CCACTAG118300.016.9773183
CACAAGA706200.015.7415477
TCGCCGG52200.014.445339145
GTTGATT112350.013.3214871
ACAAGAA872100.012.9879318
CGCCGTT24800.012.8637145
GTTTCGG84150.012.8382614
TTGATTA106900.011.7339012
CACTAGC173600.011.569224
AACCTTC773100.011.3574944
AGCTCCG138450.011.3129056
Produced by FastQC (version 0.11.2)