FastQCFastQC Report
Mon 2 Oct 2017
G23Rik_WY_1_S7_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG23Rik_WY_1_S7_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21958089
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATGT93150.022.7234973
CTCGAAT127100.021.6886231
ACGTTTA26050.020.0354884
TCGGTTT89150.019.435922
CGGGTTA35050.017.7884862
GTCGGTT107650.017.5670011
CGGTTTG97750.016.8338813
GTTCGAT69500.016.744551
CGTTTAC61150.016.59625
CCGGGTT63600.016.3537461
TGTCTAA131150.016.0290165
TCGAATG135650.015.6060022
CTCCTAT91750.014.9828361
TAAATTC152750.014.3330149
CTCCGAT145350.014.3116371
CCCGTAT50000.013.9649541
CCCGTTT53750.013.9378921
CGGTTTA22850.013.6413523
TCTAAAT172450.013.5779847
CTAAATT161050.013.4587728
Produced by FastQC (version 0.11.2)