FastQCFastQC Report
Mon 2 Oct 2017
G23Rik_WY_1_S7_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG23Rik_WY_1_S7_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21958089
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCAC73650.038.196873
CACGATC100100.029.9012131
ACGATCA125500.022.8205492
GCGTCGG83900.022.122536145
TCACAAG183100.015.9596776
TAACGCG14600.015.8937087
CCACGAT92150.015.5274361
GTTTCGG52500.015.4678334
CGTGGAT80650.015.3880481
GCCACGA64050.015.2730111
CGTTGAT29050.013.5702111
AATCGTG178750.013.3456715
GATCACA234000.012.9828184
CGTGCAT178100.012.8659088
GTTGATT85500.012.6367271
TCGTTGC105600.012.2915428
AGATATA152550.012.2624784
TTGATTA75700.012.2598972
CCACTAG83650.011.8747213
AAATCGT205800.011.8376264
Produced by FastQC (version 0.11.2)