FastQCFastQC Report
Mon 2 Oct 2017
G23Rik_WY_2_S6_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG23Rik_WY_2_S6_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22426989
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC347620.15500074486147025TruSeq Adapter, Index 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAA305650.019.473331145
GGGTTCG53200.017.5773373
TGTCTAA127150.017.3316525
CGTATCG25100.017.3281633
ACGTTTA21400.016.9367944
CTAAATT146050.015.1395168
TAAATTC146550.015.0388979
GTCCTAT64300.014.9338731
ACGTTCG18950.014.919616
TCGGTTT83750.014.6299782
GTCCGGT34400.014.3800351
TAGGTAT218050.014.3633569
TTACCGG47900.014.2267628
GTCGTTT94900.013.95131
TCTAAAT164650.013.8255257
TTAGGTA228300.013.7815338
GTCGGTT108700.013.7193581
GTCTTAG234000.013.5687855
GTCCGTT41250.013.2265571
CTTAGGT243300.013.1702467
Produced by FastQC (version 0.11.2)