FastQCFastQC Report
Mon 2 Oct 2017
G23Rik_WY_3_S5_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG23Rik_WY_3_S5_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27512589
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC665560.2419110756897506TruSeq Adapter, Index 2 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC302990.11012776732862181TruSeq Adapter, Index 2 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCTAA155450.018.7931965
ACGTTTA29450.018.707524
GGGTTCG65850.018.0541043
TAAATTC180050.017.797179
CTAAATT184150.016.2980738
TCTAAAT202000.015.6833377
TCGGTTT107600.015.3626912
GTCCGGT42300.015.13345051
GTCGTTT120950.014.314191
GTTACGG64850.014.0853937
TAGGTAT287300.013.7525339
GTCGGTT136300.013.5560621
AGTTACG94150.013.5516966
CTATCGC22050.013.4797828
GTCTTAG299150.013.3520785
ACGTTCG27250.013.3016286
TTAGGTA299100.013.2580168
TTACCGG57450.013.2497258
GTCCGGA52900.013.2011341
CGTATCG29900.013.0921353
Produced by FastQC (version 0.11.2)