FastQCFastQC Report
Mon 2 Oct 2017
G23Rik_WY_3_S5_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG23Rik_WY_3_S5_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27512589
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT483230.17563959538667917Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG299030.10868842623280564Illumina Single End PCR Primer 1 (100% over 50bp)
CACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCT283910.10319276023059844No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCAC130100.031.8785843
GCCACGA98300.028.218081
TCTAACG48050.025.8051685
CCACGAT145150.024.6420271
CACGATC186700.024.1624831
ACGATCA196900.021.7998312
CTAACGC74600.017.5938266
TAACGCG76150.017.1412667
ACGCGTG76700.016.356089
AACGCGT78100.016.1559378
TCACAAG273600.015.7961316
CCACTAG97350.015.3430443
GCGTCGG189450.014.159912145
GATCACA327400.013.6642814
TAGCATA58500.013.1394889
CACTAGC112450.013.0248344
TCACCCG122600.012.5970683
AGTCTAA108450.011.9006513
AATCGTG209850.011.8173775
ACCCGGC122750.011.7553515
Produced by FastQC (version 0.11.2)