FastQCFastQC Report
Mon 2 Oct 2017
WT_CON_1_S16_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameWT_CON_1_S16_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27148228
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTGTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAAT445320.16403280538236234No Hit
GGATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGG303830.11191522334349042No Hit
GGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTC301490.11105328863452893No Hit
CAGGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCC296360.10916366254180568No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTTT107600.020.3488032
CGGTTTG112300.018.9133323
GGGTTCG61350.018.078283
GTCCGTT43350.017.9526391
GTCGGTT144550.017.409711
TCGATTC63500.017.1247447
TGTCTTA454650.017.0762484
TTAGGTA405100.016.7323118
TAGGTAT406650.016.4908089
AGCTTTA258800.016.4139654
CTCGAAT146950.015.9869821
CTTAGGT425350.015.8845927
GTCTTAG422800.015.8766115
GTTCGAT95100.015.831551
ACTAATC50050.015.7894352
GTCGTTT103900.015.4707131
ATCATGC113600.015.2519777
TATCATG104000.015.1260316
CGAATGT115600.014.9871913
GGGCGAT139150.014.6877991
Produced by FastQC (version 0.11.2)