FastQCFastQC Report
Mon 2 Oct 2017
WT_CON_1_S16_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameWT_CON_1_S16_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27148228
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCT466520.1718417865062869No Hit
GGAAACCTTCCAGCTGATGGGGCTCTATGGCCGAGAACCAGATTTGAGTT424760.15645956708482042No Hit
CTTTAATGTAGAAAAGATTAATGGGGAATGGCATACTATTATCCTGGCCT368520.13574366621644698No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCACGA99750.041.568681
CGATCAC171500.041.0518573
CACGATC178700.040.6879271
ACGATCA194950.036.1882932
CCACGAT133900.030.4322622
TCACAAG295600.024.113736
GATCACA356950.020.3941144
GCGTCGG100700.018.432158145
CCACTAG105300.018.384873
TCTAACG19150.015.9031465
CGTTGAT29750.015.2137221
CGCCGTT22800.014.946127145
GTTGATT86600.014.7520021
ATCACAA541950.014.1154835
CACTAGC137750.013.790764
TCGCCGG47250.013.503507145
TAACGCG24950.013.0791077
GTTTCGG75800.013.0091294
TTGATTA93150.012.8433872
CACAAGA603300.012.3565127
Produced by FastQC (version 0.11.2)