FastQCFastQC Report
Mon 2 Oct 2017
WT_CON_2_S15_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameWT_CON_2_S15_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22873700
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCT414340.18114253487629897No Hit
CTTTAATGTAGAAAAGATTAATGGGGAATGGCATACTATTATCCTGGCCT361130.1578800106672729No Hit
GGAAACCTTCCAGCTGATGGGGCTCTATGGCCGAGAACCAGATTTGAGTT332780.1454858636774986No Hit
CCAGATTTGAGTTCAGACATCAAGGAAAGGTTTGCACAACTATGTGAGGA277700.1214058066687943No Hit
CCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCC231630.1012647713312669No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGATC152700.042.402441
CGATCAC154900.040.770283
ACGATCA164950.038.33022
GCCACGA79400.037.50991
CCACGAT115050.036.5461921
TCACAAG258550.024.7364886
GATCACA301650.021.5127854
CGCCGTT17300.020.954947145
GCGTCGG88600.020.785606145
CCACTAG92100.020.0750893
TCTAACG18200.018.326785
GTTTCGG76100.016.1019694
AGCTCCG97900.015.7024396
CACTAGC120750.014.951654
TCCGGGG110800.014.5947349
TAACGCG25100.014.1564827
ATCACAA477350.013.823065
TAGATCC130750.013.53084853
CTTAGAT119550.013.3110741
GCTCCGG115750.013.2815227
Produced by FastQC (version 0.11.2)