FastQCFastQC Report
Mon 2 Oct 2017
WT_CON_3_S14_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameWT_CON_3_S14_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27602733
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATT332780.1205605256551951No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATGT113950.022.6473773
CTCGAAT158500.021.8915121
GTCCGCC98950.021.320915145
TCGGTTT109650.018.5136222
CGCACAA100700.018.430584145
CTCCTAT113650.018.3695741
CGCACAC247950.017.806631145
ATTGGAC324550.016.4844136
TCGATAC38000.016.2172552
CGGTTTG114400.016.1583253
TCCGATT129250.015.20132
AATTGGA394700.014.8213535
CGCCTAT19700.014.7699971
GTCGGTT141600.014.6922881
CTGGAAT582300.014.6159051
CTCCGGT95400.014.4111981
AGCTTTA268050.014.3331724
CTTCGAT79250.014.3189761
TTCGATT94050.014.2611632
CGCCATT59250.014.2415191
Produced by FastQC (version 0.11.2)