FastQCFastQC Report
Mon 2 Oct 2017
WT_CON_3_S14_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameWT_CON_3_S14_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27602733
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCT415620.1505720466158188No Hit
CTTTAATGTAGAAAAGATTAATGGGGAATGGCATACTATTATCCTGGCCT337910.12241903727431627No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCAC146950.041.5446433
CACGATC170700.037.4616621
ACGATCA186100.032.9217452
CCACGAT132200.031.1984831
CCACTAG122300.027.1526093
TCACAAG269500.023.1392026
CACTAGC163350.020.01854
GATCACA335700.018.7906594
GCCACGA88450.018.6520711
GCGTCGG117450.018.581083145
TCTAACG23950.018.1651735
ACTAGCC192550.017.2094295
GTTTCGG83050.015.8893394
TAGCCTC212600.015.4512977
CCCACTA219250.015.1790862
CCCCACT285400.013.8375671
TTGAGTT526600.013.7980227
TCGCCGG49950.013.644258145
TTTGAGT552900.013.429576
CGCCGTT22500.013.211706145
Produced by FastQC (version 0.11.2)