FastQCFastQC Report
Mon 2 Oct 2017
WT_WY_1_S13_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameWT_WY_1_S13_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21628895
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCT295320.1365395689423801No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCAC98800.043.6648333
CACGATC115900.038.80691
ACGATCA143800.030.252562
GCGTCGG71100.023.556248145
CCACGAT100800.022.8171671
TCACAAG202950.021.6516766
GCCACGA64400.018.4804881
GATCACA250200.018.0249844
GTTTCGG52500.015.1916594
ATCACAA323900.014.73020655
CGTGGAT82700.014.2145151
AGATATA146400.013.5700454
GTTGATT77350.013.3097671
AATCGTG160100.012.9076575
TTGATTA76500.012.7950532
TCGTTGC94800.012.4680428
CCACTAG83900.012.4443533
CGTGCAT163800.012.3954748
GATATAC94450.012.3599825
ATATACC96250.012.2045636
Produced by FastQC (version 0.11.2)