FastQCFastQC Report
Mon 2 Oct 2017
WT_WY_2_S12_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameWT_WY_2_S12_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24537441
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAT138950.022.7801571
CGAATGT101400.021.3750633
ACGTTTA26450.020.006514
CGGGTTA38800.019.2463252
CTCCTAT97400.018.0046251
TCGGTTT92650.017.5285052
CCGGGTT74350.016.7355941
CGTTTAC63100.016.083385
CTCCGAT148400.015.9848931
TCGAATG141500.015.4224152
GTCCGTT46650.015.130251
GTTCGCA148700.014.5279176
GTCGGTT113250.014.4567391
CTAGCGG70450.014.406298
CCCGTTT67300.014.3801221
TCCGATT107500.014.3652582
TGTCTAA142050.014.2377565
GTTCGAT71700.014.2080631
CTCGTTT112850.014.1211011
AGTTCGC145500.013.9001595
Produced by FastQC (version 0.11.2)