FastQCFastQC Report
Mon 2 Oct 2017
WT_WY_2_S12_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameWT_WY_2_S12_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24537441
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCAC79900.035.2089843
CACGATC105100.028.3430481
GCGTCGG97350.022.790182145
ACGATCA135250.021.4432832
CCACGAT103000.019.3514961
TCACAAG202800.014.15916
AGATATA160100.013.5409284
GTTTCGG60250.012.8764254
GATATAC98900.012.7569085
ATATACC93950.012.6577196
CGTGGAT93250.012.2141961
GATCACA253900.011.9366414
AATCGTG179150.011.8183955
CGTTGAT36150.011.5121291
GCCACGA72350.011.5041741
TCGCCGG56800.011.48832145
CGTGCAT183650.011.45035658
CGTTAAT15550.011.26861
ATTTCGC55150.011.1758826
TAGATCC85750.011.1611353
Produced by FastQC (version 0.11.2)