FastQCFastQC Report
Mon 2 Oct 2017
WT_WY_3_S11_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameWT_WY_3_S11_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23102892
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATGT100000.021.5295123
CTCGAAT139000.019.5680981
TCGGTTT101700.018.9622212
TGTCTAA139700.017.9540775
GTCGGTT118800.016.8348031
CGGGTTA38200.016.7012042
TAAATTC156500.016.5840519
CTCCTAT104400.016.579381
CGGTTTG110300.016.1672943
ACGTTTA31750.015.9820344
GTTCGAT76650.015.845141
TCGGAAA101500.015.71384145
TCGAATG138400.015.2959082
GTCTAAA176300.015.255346
CGTTTAC71550.015.0959345
GGGTTCG54250.014.8320443
ACGATAA36550.014.677478
GTCCGGT34450.014.5663821
CCGGGTT73000.014.545271
GTCGCTT40750.014.2775661
Produced by FastQC (version 0.11.2)