FastQCFastQC Report
Mon 3 Dec 2018
G166-M13.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG166-M13.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50000000
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAG541080.10821599999999999No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA88500.032.4487461
ATCGGAA97500.029.830722
TACACGA86200.028.3546742
CACGACG88500.027.71720144
CTACACG93950.026.24983841
CGTATGC51200.025.13643643
TCGGAAG125350.023.413333
TCGTATG46700.020.77492542
ACACGAC124750.019.98053643
CGGAAGA226750.018.9678691
CCGTCCG86550.018.91139840
CGTCTGA220800.018.78113413
GACGCAA58200.018.5223874
ACGTCTG224150.018.5102412
CTCGTAT74850.018.36986544
TCGCGTA20150.018.12386543
AGTCCGC58800.017.99639742
AACCCGT92250.017.93331537
ACACGTC235800.017.68901410
AGTCTAC150450.017.66378825
Produced by FastQC (version 0.11.2)